Product description: Silantes SILAC RPMI Lysine(4) Arginine(10) Kit

Content of the kit:

- 2 x SILAC RPMI (Dulbecco"s Modified Eagle Medium) without L-Glutamine, L-Arginine and L-Lysine, amount: 500 mL

- 2 x L-Glutamine, isotopic labelling: unlabelled, amount: 5 mL , in solution, concentration: c=0.2 mol/L, molecular weight = 146.1 g/mol,chemical formula: C₅H₁₀N₂O₃, chemical purity > 95 %

- 2 x SILAC FBS (Fetal Bovine Serum, dialyzed), amount: 50 mL

- 1 x L-Lysine(4) 2HCl, isotopic labelling: ²H₄, amount: 500 µL, in solution, concentration: c=0.274 mol/L,molecular weight= 223.1 g/mol, chemical formula: C₆D₄H₁₀N₂O₂ 2HCl, isotopic enrichment: > 98 atom %,chemical purity: > 95 %

- 1 x L-Arginine(10) HCl, isotopic labelling: ¹³C¹⁵N, amount: 500 µL, in solution, concentration: c=1.15mol/L, molecular weight=220.7 g/mol, chemical formula: ¹³C₆H₁₄¹⁵N₄O₂ HCl, isotopic enrichment: > 98 atom %, chemical purity: > 95%

- 1 x L-Lysine(0), isotopic labelling: unlabelled, amount: 500 µL, in solution, concentration: c=0.274 mol/L, molecular weight=182.65g/mol, chemical formula: C₆H₁₄N₂O₂ HCl

- 1 x L-Arginine(0), isotopic labelling: unlabelled, amount: 500 µL, in solution, concentration: c=1.15 mol/L, molecular weight= 210.66g/mol, chemical formula: C₆H₁₄N₄O₂ HCl

All products are sterile.

Dry ice shipment required.

The SILAC-procedure in short:

Cells are grown in culture media that are identical with exception of amino acids. The “light” culture contains all the amino acids in unlabelled form andthe “heavy” culture contains one or two amino acids in labelled form. The labelled amino acids are usually L-Lysine labelled with ²H(D4),¹³C or ¹³C¹⁵N and/or arginine labelled with ¹³C or ¹³C¹⁵N. Thelabelled amino acids increase the molecular weight allowing identification and quantification of the proteins/peptides stemming from culture light and heavy,respectively.