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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
silantes/SILAC RPMI L-Lysine(4) L- Arginine(10) Kit/284996424
产品编号:284996424
市  场 价:¥12400.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$620.00
品      牌: silantes
公      司:silantes
公司分类:
silantes/SILAC RPMI L-Lysine(4) L- Arginine(10) Kit/284996424
商品介绍
  • Description

Product description: Silantes SILAC RPMI Lysine(4) Arginine(10) Kit

Content of the kit:

- 2 x SILAC RPMI (Dulbecco"s Modified Eagle Medium) without L-Glutamine, L-Arginine and L-Lysine, amount: 500 mL

- 2 x L-Glutamine, isotopic labelling: unlabelled, amount: 5 mL , in solution, concentration: c=0.2 mol/L, molecular weight = 146.1 g/mol,chemical formula: C5H10N2O3, chemical purity > 95 %

- 2 x SILAC FBS (Fetal Bovine Serum, dialyzed), amount: 50 mL

- 1 x L-Lysine(4) 2HCl, isotopic labelling: 2H4, amount: 500 µL, in solution, concentration: c=0.274 mol/L,molecular weight= 223.1 g/mol, chemical formula: C6D4H10N2O2 2HCl, isotopic enrichment: > 98 atom %,chemical purity: > 95 %

- 1 x L-Arginine(10) HCl, isotopic labelling: 13C15N, amount: 500 µL, in solution, concentration: c=1.15mol/L, molecular weight=220.7 g/mol, chemical formula: 13C6H1415N4O2 HCl, isotopic enrichment: > 98 atom %, chemical purity: > 95%

- 1 x L-Lysine(0), isotopic labelling: unlabelled, amount: 500 µL, in solution, concentration: c=0.274 mol/L, molecular weight=182.65g/mol, chemical formula: C6H14N2O2 HCl

- 1 x L-Arginine(0), isotopic labelling: unlabelled, amount: 500 µL, in solution, concentration: c=1.15 mol/L, molecular weight= 210.66g/mol, chemical formula: C6H14N4O2 HCl

All products are sterile.

Dry ice shipment required.

The SILAC-procedure in short:

Cells are grown in culture media that are identical with exception of amino acids. The “light” culture contains all the amino acids in unlabelled form andthe “heavy” culture contains one or two amino acids in labelled form. The labelled amino acids are usually L-Lysine labelled with 2H(D4),13C or 13C15N and/or arginine labelled with 13C or 13C15N. Thelabelled amino acids increase the molecular weight allowing identification and quantification of the proteins/peptides stemming from culture light and heavy,respectively.

品牌介绍

Silantes由Hermann Heumann 博士创建于1998年创立,总部位于德国慕尼黑。Silantes公司能够提供高丰度稳定性同位素(2H, 13C及15N)标记的生物分子, 如氨基酸、核苷酸、多肽、蛋白质和DNA。Silantes以优惠的价格提供高质量产品及创新产品的开发,并为客户提供灵活的、面向解决方案的服务,受到世界各地各级相关科研机构广泛好评。


Silantes技术

创始人开发了一种基于化学无机自养细菌生产同位素标记生物分子的新方法(专利申请中)。这项技术利用了化学自养营养细菌不仅拥有类似于绿色植物代谢无机碳的优点,同时具有细菌更高效、更可靠及能够表达异源蛋白质的优势。采用被标记的化学无机自养细菌生产稳定同位素标记的生物分子,能够提供优异的一致性的同位素标记的生物分子,特别是三重标记(2H、13C和15N)的生物分子。

Silantes产品

    Silantes提供高丰度稳定性同位素(2H, 13C及15N)标记的生物分子。

  • ribo-Nucleosidetriphosphates (rNTPs)

  • desoxy-Nucleosidetriphosphates (dNTPs)

  • Rich growth media for E. coli

  • Rich growth media for yeast

  • Amino Acids

  • Bio-NMR standards (Ubiquitin)

  • Mice feed for in vivo labelling

  • 13C-Lys-specific labelled diet for SILAC

  • 15N-uniform labelled diet for SILAM


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